SECTION 252:690-3-29. WET testing methods  


Latest version.
  •   The specific tests and test organisms used for determining whole effluent toxicity include:
    (1)   Acute test/D. pulex. Acute 48-hour static renewal toxicity test using Daphia pulex (Method 2021.0) as described in EPA publication no. 821-R-02-012 (October 2002),Fifth Edition, Methods for Measuring the Acute Toxicity of Effluent to Freshwater and Marine Organisms.
    (2)   Acute test/P. promelas. Acute 48-hour static renewal toxicity test using Pimephales promelas (Method 2000.0) as described in EPA publication no. 821-R-02-012 (October 2002), Fifth Editon, Methods for Measuring the Acute Toxicity of Effluent to Freshwater and Marine Organisms.
    (3)   Chronic test/C. dubia. Chronic 7-day static renewal survival and reproduction test using Ceriodaphnia dubia (Method 1002.0), as described in EPA publication no. 821-R-02-013 (October 2002), Fourth Edition, Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms.
    (4)   Chronic test/P. promelas. Chronic 7-day static renewal larval survival and growth test using Pimephales promelas (fathead minnow) (Method 1000.0), as described in EPA publication no. 821-R-02-013 (October 2002), Fourth Edition, Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms.
    (5)   Acute test/D. Magna. Acute 48-hour static renewal toxicity test using Daphnia magna (Method 2021.0), as described in EPA Publication No. 821-R-02-012 (October, 2002), fifth edition, Methods for Measuring the Acute Toxicity Of Effluent to Freshwater and Marine Organisms.
    (6)   Chronic test/D. Magna. Chronic 21-day static renewal life-cycle toxicity test using Daphnia magna, (ASTM Designation E-1193), as described in ASTM Publication E-1193-97 (Reapproved 2004), Standard Guide for Conducting Daphnia Magna Life Cycle Toxicity Tests.
    (7)   Acute test/Mussels. Acute toxicity test using mussels (ASTM Designation E-2455) as described in ASTM Publication E-2455-06 (Approved April, 2006), Standard Guide for Conducting Laboratory Toxicity Test with Freshwater Mussels.
[Source: Added at 19 Ok Reg 1721, eff 6-13-02; Amended at 22 Ok Reg 1477, eff 6-15-05; Amended at 24 Ok Reg 1542, eff 6-15-07]